PCC/ CCOG / MLT

Course Content and Outcome Guide for MLT 230

Course Number:
MLT 230
Course Title:
Body Fluids
Credit Hours:
3
Lecture Hours:
30
Lecture/Lab Hours:
0
Lab Hours:
0
Special Fee:
 

Course Description

Introduces the composition, testing procedures, and the clinical correlation of results for cerebrospinal, synovial, pleural, pericardial, seminal, amniotic, and peritoneal fluids. Presents laboratory mathematics, autoimmune diseases, special urine testing protocol surrounding the aminoacidurias and porphyrinurias, and fecal fat analysis and its clinical significance. Acceptance into the second year of the MLT Program required. Audit available.

Addendum to Course Description

The anatomy and physiology of the urinary system and the normal and abnormal composition of urine will be reviewed and expanded. In addition, the normal and abnormal composition of cerebrospinal, synovial, pericardial, plural, seminal, and peritoneal fluids fluid will be reviewed. Laboratory testing of body fluids for normal and abnormal constituents, sources of error, and clinical correlation with various disease states will be presented.

Intended Outcomes for the course

Upon successful completion students should be able to:

1. Use an understanding of the normal and abnormal constituents of body fluids and their correlation with appropriate pathologic conditions to make appropriate and effective on-the-job professional decisions.
2. Apply appropriate laboratory techniques, methodologies, instruments and equipment; and accurately calculate, record, and tabulate data to improve patient care.
3. Adapt laboratory techniques and procedures in a corrective manner when errors and discrepancies in results are obtained to affect resolution in a professional and timely manner.

Course Activities and Design

The material in this course will be presented in a traditional classroom (Campus program), or distance-learning format (Distance learning program).  Both traditional and distance learning sections include required laboratory activities.  Traditional classroom instruction consists of lecture, discussion, web based instructional media (tutorials, animations, videos, etc.) and other educationally sound practices. Distance learning sections include similar educational practices and are primarily presented in a web based format. Both campus and distance learning sections require periodic campus based laboratory exercises.  In addition, the distance learning section may have hospital based laboratory exercises.  A laboratory manual will be provided.  The laboratory exercises provide an opportunity for students to employ the testing principles and methodologies discussed and allow for discussion, application and correlation of the concepts presented.  The student is expected to perform all laboratory procedures according to the criteria established by the department.

Outcome Assessment Strategies

The instructor will outline the methods used to evaluate student progress and the criteria for assigning a grade at the beginning of the course. Outcome assessment strategies may include written and practical examinations and laboratory write-ups. Evaluation of problem solving skills, teamwork, attendance, laboratory performance and communication skills may also be included. Extra credit may be available at the instructor€™s discretion.

Course Content (Themes, Concepts, Issues and Skills)

  • Course Themes
    • Theme 1: Cerebrospinal, Synovial, Pericardial, Pleural, Peritoneal, amniotic, and Seminal Fluid
      • Anatomy and physiology associated with each body fluid
      • Methods for body fluid collection and processing
      • Composition of each body fluid
      • Common tests performed on each body fluid
      • Preparation of cytocentrifuged specimens
      • Recognizing normal and abnormal results and interpreting its clinical significance
    • Theme 2: Autoimmune Diseases
      • Pathophysiology of autoimmnune disease
      • ANA testing
    • Theme 3: Laboratory Mathematics
      • Unit  conversion
      • Calculations associated with solutions
    • Theme 4: Special Urine Testing
      • General biochemical pathway of porphyrin and heme production
      • Aminoacidurias, porphyrias and other common inborn errors of metabolism
    • Theme 3: Fecal Fat
      • Steatorrhea causes and symptoms
      • Testing procedures for both qualitative and quantitative fecal fat analysis

 

  • Laboratory Skills
    • Use a bright field light microscope to view and interpret slides, including
      • Correctly setting up and focusing the microscope
      • Proper handling, cleaning, and storage of the microscope
      • Correct use of all lenses
      • Performing differential counts in body fluid smears
      • Identifying and differentiating leukocyte and other body fluid cells
      • Recording microscopic observations and interpreting results
    • Troubleshooting and problem solving
      • Recognizing errors or discrepancies in results during lab procedures
      • Selecting corrective actions for problem solving

 

  • Cognitive Skills
    • Cognitive processes, including
      • Formulating a clear, answerable question
      • Predicting expected results
      • Following written protocols and verbal instructions
    • Analysis skills, including
      • Collecting and organizing data in a systematic fashion
      • Presenting data in an appropriate form
      • Assessing the validity of the data (including integrity and significance)
      • Recognizing errors and developing a corrective course of action
      • Drawing appropriate conclusions based on the results
    • Communication skills, including
      • Discussing and presenting lab results or findings in the laboratory
    • Interpersonal and citizenry skills, including
      • Working effectively in teams or groups so that the task, results, and analysis may be shared
      • Effectively managing time and tasks allowing concurrent and/or overlapping tasks to be done simultaneously, by individuals and/or within a group
      • Integrating knowledge and making informed judgments about pathologic conditions diagnosed and monitored through body fluid analysis in the clinical setting and in everyday life

 

  • Laboratory Safety
    • Laboratory procedures, including
      • Reporting all spills and broken glassware to the instructor and receiving instructions for clean up
      • Methods for aseptic transfer
      • Minimizing or containing the production of aerosols and describing the hazards associated with aerosols
      • Washing hands prior to and following laboratories and at any time contamination is suspected
      • Using universal precautions with blood and other body fluids and following the requirements of the OSHA Bloodborne Pathogen Standard
      • Disinfecting lab benches and equipment prior to and at the conclusion of each lab session, using an appropriate disinfectant and allowing a suitable contact time
      • Identification and proper disposal of different types of waste\
      • Good lab practice, including returning materials to proper locations, proper care and handling of equipment, and keeping the bench top clear of extraneous materials
    • Protective procedures, including
      • Tying long hair back, wearing personal protective equipment (eye protection, coats, gloves, closed shoes), and using such equipment in appropriate situations
      • Always using appropriate pipetting devices and understanding that mouth pipetting is forbidden
      • Never eating or drinking in the laboratory
      • Never applying cosmetics, handling contact lenses, or placing objects (fingers, pencils, etc.) in the mouth or touching the face
    • Emergency procedures, including
      • Locating and properly using emergency equipment (eye wash stations, first aid kits, fire extinguishers, chemical safety showers)
      • Reporting all injuries immediately to the instructor
      • Following proper steps in the event of an emergency