Portland Community College | Portland, Oregon

Course Number:
BIT 201
Course Title:
Immunochemical Methods
Credit Hours:
Lecture Hours:
Lecture/Lab Hours:
Lab Hours:
Special Fee:

Course Description

Introduces the general properties and uses of antibody molecules. Includes an overview immune response, biosynthesis of immunoglobulin, obtaining, purifying and labeling antibodies, and using antibodies in a variety of common applications (ELISA, Western blot, immunoprecipitation and immunocytochemistry, antibody-based affinity chromatography). Prerequisites: BIT 109 or 110; BI 112 or CH 100 or higher; or instructor permission.

Intended Outcomes for the course

Upon successful completion students should be able to:Plan, carry out and interpret the results of several different immunochemical procedures using standard laboratory protocols. These should include, but not be limited to, Ab purification, ELISA and western blot. Communicate the methods and results of laboratory work in the form of standard scientific reports, oral presentation and the laboratory notebook.